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Laboratory
Substrate preparation and storage areas Sawdust can be purchased in bulk from either a sawmill, or from sawdust wholesalers, the latter usually can supply it packaged in paper bags either slightly compacted or loose, for a higher price. In both cases you will have to make sure that you are getting hardwood sawdust. If you get it in bulk an outside storage area will be acceptable but it must be covered. Bags can be stored indoor in the same area as the wheat, or rice bran that you will need as a supplement. Other supplies will include limestone and gypsum. The substrate preparation area will be where the above materials are mixed and water added and finally bagged to be sterilized. Equipment for this area will include.
Sterilization area The bags of substrate (mushroom growing media) will have to be sterilized to get rid of competitors such as molds and bacteria. This will provide you with a clean material for your mushrooms to grow on. This is done with steam, either at low pressure for up to 18 hours in a "steam box" such as a converted 20' shipping container, or at high pressure in an autoclave for 4-6 hours. Equipment here will be:
Cool down and bag inoculation area Ideally this room is directly connected to your exit door of the double door autoclave or steam room, and it is well sealed with an incoming HEPA filtered air flow. The sterilized bags should be brought down to inoculation temperature as quickly as possible (ideally 8 hrs), so a cooling unit in this area is necessary. Once cooled down the bags should be inoculated as soon as possible and must be done on a workbench in front of a HEPA filter This area is very important as this is where the spawn is introduced into the sterile media in the bags, lax procedures here will result in unacceptable levels of contamination (more than 3-5 %). Equipment here will include:
Block incubation or spawn run area The inoculated bags will require a spawn run period ranging from 2-4 months depending on your shiitake strain. At Fun Guy Farm we use a 4 month, long incubation strain, this has proven to give the best quality mushroom an the least contamination of the blocks from molds. The bags will have to be kept between 20-23C (68-75F) and 70-80% humidity throughout the four months. They should be arranged on shelves so that they do not touch and the air in the room should be exchanged often to allow the dissipation of the carbon dioxide produced by the mycelium as it takes over the substrate. The size of this area will depend on the length of your spawn run, for a four month time period multiply your weekly bags production by 16, i.e: 1000 bags per week x 16 weeks= 16000 bags. As you can see this area will have to be quite large, to be able to exercise more control it is best to have several rooms, 4 rooms or one for each month of production is recommended. Equipment here will be:
Fruiting rooms This is where payoff happens, the now solid blocs of mycelium and sawdust are removed from the plastic bags and put on shelves to allow for the mushroom s to pop out. The blocks can produce for a period of up to six months, the first 3 flushes will be the most productive, tapering off for flushes 4-6. Some people do not even bother with the last 2 if they are short on space. The environmental requirements for the blocks change somewhat from the beginning to the end of the cycle, so to be able to control these changes it is advisable to have at least 4 grow rooms so that blocks at the some stage of development are not mixed with older blocks. This is not an absolute necessity as a one grow room operation can work. Equipment for the grow rooms will include:
Fresh mushrooms sorting and storage area The fresh mushrooms need to be stored at 3-4C (35F) and will keep a couple of weeks at this temperature. They should be delivered as soon as possible however as they will also sit with the wholesaler and then the retailer. They will be sorted into the various grades and weighed and boxed or packaged. A mushroom drier my be required if there are periodic or seasonal gluts in your area, or if you have a market for dried mushrooms. Equipment required here is:
We can assist you on all phases of indoor cultivation. The most important phase is the initial set up. With proper consultation expensive mistakes can be avoided and will lead to higher yields as well as progressive schedule rather than one that is marred with down time to modify environments.
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